The aim of our work is to define endogenous mono-ADP-ribosylation-dependent pathways in cell physiology and pathology. Recently, we devised a new methodology based on the use of the macro domain as a powerful tool that specifically recognizes this post-translational modification on cellular proteins. We are presently involved in studies focused on members of two different families of mono-ADP-ribosyltransferases: the well characterized arginine-specific ARTC ecto-enzymes; and some members of the PARP/ARTD family.
Specifically, we are focused on the study of:
- Mono-ADP-ribosylation-dependent signalling mechanisms associated with the endoplasmic reticulum stress response and cancer development.
- Role of the ARTD15-dependent mono-ADP ribosylation of karyopherin-beta1 in the nucleocytoplasmic trafficking.
- Development of ARTD15 specific inhibitors.